Response of antibiotic and heavy metal resistance genes to two different temperature sequences in anaerobic digestion of waste activated sludge

Hyun Min Jang; Jangwoo Lee; Sangki Choi; Jingyeong Shin; Eunsung Kan; Young Mo Kim

doi:10.1016/j.biortech.2018.07.051

Response of antibiotic and heavy metal resistance genes to two different temperature sequences in anaerobic digestion of waste activated sludge

Hyun Min Jang
, Jangwoo Lee
, Sangki Choi
, Jingyeong Shin
, Eunsung Kan
, Young Mo Kim^*

^*Corresponding author for this work

Environmental Engineering

Gwangju Institute of Science and Technology
Texas A&M AgriLife
Swiss Federal Institute of Aquatic Science and Technology
Tarleton State University

Research output: Contribution to journal › Journal article › peer-review

54 Scopus citations

Abstract

Response of antibiotic resistance genes (ARGs) and heavy metal resistance genes (HMRGs) to two different temperature sequences (i.e., mesophilic-thermophilic and thermophilic-mesophilic) were investigated. Higher removal of total ARGs (twenty-one targeted subtypes) and HMRGs (three targeted subtypes) was achieved by the mesophilic-thermophilic sequence than by the thermophilic-mesophilic sequence. The sequence of mesophilic-thermophilic showed the highest removal of total ARGs, but the sequence of thermophilic-mesophilic proved more suitable for removal of class 1 integrons (intI1). Correlation analysis indicated that intI1 correlated significantly with tetG, tetQ, tetX, sul2, aac(6′)-lb-cr, bla_TEM, ermB and floR. High-throughput sequencing revealed that the mesophilic-thermophilic sequence TPAD removed more human bacterial pathogens (HBPs) than did the thermophilic-mesophilic sequence. Also, significantly positive correlation was observed between ARGs and HBPs. For instance, Mycoplasma pneumonia showed significantly positive correlation with several ARGs including tetE, tetQ, tetX, tetZ, sul1, sul2, aac(6′)-lb-cr and floR.

Original language	English
Pages (from-to)	303-310
Number of pages	8
Journal	Bioresource Technology
Volume	267
DOIs	https://doi.org/10.1016/j.biortech.2018.07.051
State	Published - 2018.11

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being
SDG 6 Clean Water and Sanitation

Keywords

Antibiotic resistance genes (ARGs)
Heavy metal resistance genes (HMRGs)
High-throughput sequencing
Human bacterial pathogens (HBPs)
Temperature phased anaerobic digestion (TPAD)

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10.1016/j.biortech.2018.07.051

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@article{824f407a332d4e1b99b23e01af86e346,

title = "Response of antibiotic and heavy metal resistance genes to two different temperature sequences in anaerobic digestion of waste activated sludge",

abstract = "Response of antibiotic resistance genes (ARGs) and heavy metal resistance genes (HMRGs) to two different temperature sequences (i.e., mesophilic-thermophilic and thermophilic-mesophilic) were investigated. Higher removal of total ARGs (twenty-one targeted subtypes) and HMRGs (three targeted subtypes) was achieved by the mesophilic-thermophilic sequence than by the thermophilic-mesophilic sequence. The sequence of mesophilic-thermophilic showed the highest removal of total ARGs, but the sequence of thermophilic-mesophilic proved more suitable for removal of class 1 integrons (intI1). Correlation analysis indicated that intI1 correlated significantly with tetG, tetQ, tetX, sul2, aac(6′)-lb-cr, blaTEM, ermB and floR. High-throughput sequencing revealed that the mesophilic-thermophilic sequence TPAD removed more human bacterial pathogens (HBPs) than did the thermophilic-mesophilic sequence. Also, significantly positive correlation was observed between ARGs and HBPs. For instance, Mycoplasma pneumonia showed significantly positive correlation with several ARGs including tetE, tetQ, tetX, tetZ, sul1, sul2, aac(6′)-lb-cr and floR.",

keywords = "Antibiotic resistance genes (ARGs), Heavy metal resistance genes (HMRGs), High-throughput sequencing, Human bacterial pathogens (HBPs), Temperature phased anaerobic digestion (TPAD)",

author = "Jang, \{Hyun Min\} and Jangwoo Lee and Sangki Choi and Jingyeong Shin and Eunsung Kan and Kim, \{Young Mo\}",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier Ltd",

year = "2018",

month = nov,

doi = "10.1016/j.biortech.2018.07.051",

language = "English",

volume = "267",

pages = "303--310",

journal = "Bioresource Technology",

issn = "0960-8524",

}

TY - JOUR

T1 - Response of antibiotic and heavy metal resistance genes to two different temperature sequences in anaerobic digestion of waste activated sludge

AU - Jang, Hyun Min

AU - Lee, Jangwoo

AU - Choi, Sangki

AU - Shin, Jingyeong

AU - Kan, Eunsung

AU - Kim, Young Mo

PY - 2018/11

Y1 - 2018/11

N2 - Response of antibiotic resistance genes (ARGs) and heavy metal resistance genes (HMRGs) to two different temperature sequences (i.e., mesophilic-thermophilic and thermophilic-mesophilic) were investigated. Higher removal of total ARGs (twenty-one targeted subtypes) and HMRGs (three targeted subtypes) was achieved by the mesophilic-thermophilic sequence than by the thermophilic-mesophilic sequence. The sequence of mesophilic-thermophilic showed the highest removal of total ARGs, but the sequence of thermophilic-mesophilic proved more suitable for removal of class 1 integrons (intI1). Correlation analysis indicated that intI1 correlated significantly with tetG, tetQ, tetX, sul2, aac(6′)-lb-cr, blaTEM, ermB and floR. High-throughput sequencing revealed that the mesophilic-thermophilic sequence TPAD removed more human bacterial pathogens (HBPs) than did the thermophilic-mesophilic sequence. Also, significantly positive correlation was observed between ARGs and HBPs. For instance, Mycoplasma pneumonia showed significantly positive correlation with several ARGs including tetE, tetQ, tetX, tetZ, sul1, sul2, aac(6′)-lb-cr and floR.

AB - Response of antibiotic resistance genes (ARGs) and heavy metal resistance genes (HMRGs) to two different temperature sequences (i.e., mesophilic-thermophilic and thermophilic-mesophilic) were investigated. Higher removal of total ARGs (twenty-one targeted subtypes) and HMRGs (three targeted subtypes) was achieved by the mesophilic-thermophilic sequence than by the thermophilic-mesophilic sequence. The sequence of mesophilic-thermophilic showed the highest removal of total ARGs, but the sequence of thermophilic-mesophilic proved more suitable for removal of class 1 integrons (intI1). Correlation analysis indicated that intI1 correlated significantly with tetG, tetQ, tetX, sul2, aac(6′)-lb-cr, blaTEM, ermB and floR. High-throughput sequencing revealed that the mesophilic-thermophilic sequence TPAD removed more human bacterial pathogens (HBPs) than did the thermophilic-mesophilic sequence. Also, significantly positive correlation was observed between ARGs and HBPs. For instance, Mycoplasma pneumonia showed significantly positive correlation with several ARGs including tetE, tetQ, tetX, tetZ, sul1, sul2, aac(6′)-lb-cr and floR.

KW - Antibiotic resistance genes (ARGs)

KW - Heavy metal resistance genes (HMRGs)

KW - High-throughput sequencing

KW - Human bacterial pathogens (HBPs)

KW - Temperature phased anaerobic digestion (TPAD)

UR - https://www.scopus.com/pages/publications/85049871105

U2 - 10.1016/j.biortech.2018.07.051

DO - 10.1016/j.biortech.2018.07.051

M3 - Journal article

C2 - 30029175

AN - SCOPUS:85049871105

SN - 0960-8524

VL - 267

SP - 303

EP - 310

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Response of antibiotic and heavy metal resistance genes to two different temperature sequences in anaerobic digestion of waste activated sludge

Abstract

UN SDGs

Keywords

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