Response surface methodology based optimization of keratinase production from alkali-treated feather waste and horn waste using Bacillus sp. MG-MASC-BT

Muthusamy Govarthanan; Thangasamy Selvankumar; Kandasamy Selvam; Chinnappan Sudhakar; Vincent Aroulmoji; Seralathan Kamala-Kannan

doi:10.1016/j.jiec.2014.12.022

Response surface methodology based optimization of keratinase production from alkali-treated feather waste and horn waste using Bacillus sp. MG-MASC-BT

Muthusamy Govarthanan^*
, Thangasamy Selvankumar
, Kandasamy Selvam
, Chinnappan Sudhakar
, Vincent Aroulmoji
, Seralathan Kamala-Kannan

^*Corresponding author for this work

Environmental Biotechnology

Periyar University
Jeonbuk National University

Research output: Contribution to journal › Journal article › peer-review

34 Scopus citations

Abstract

A native feather-degrading keratinolytic bacterium, Bacillus sp. MG-MASC-BT was isolated from feather dumping soil in Mallasamudram, Tamil Nadu, India and screened for keratinase production using alkali treated horn waste (HW) and feather waste (FW). The study factors influencing keratinase production was optimized by Box-Behnken design (BBD). The maximum enzyme production (1075. U/ml) was observed at pH 7.0, temperature 55. °C and growth period of 60. h. The media supplemented with 6% of HW and FW enhanced keratinase production. Statistics based contour plots were generated to evaluate the changes in the response surface and to understand the relationship between the enzyme yield and the culture conditions.

Original language	English
Pages (from-to)	25-30
Number of pages	6
Journal	Journal of Industrial and Engineering Chemistry
Volume	27
DOIs	https://doi.org/10.1016/j.jiec.2014.12.022
State	Published - 2015.07.25

Keywords

Bacillus sp
Feather
Horn
Keratinase
Solid state fermentation

Quacquarelli Symonds(QS) Subject Topics

Engineering - Chemical

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10.1016/j.jiec.2014.12.022

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@article{019f2cfba39d48d5888cc53180ae0b66,

title = "Response surface methodology based optimization of keratinase production from alkali-treated feather waste and horn waste using Bacillus sp. MG-MASC-BT",

abstract = "A native feather-degrading keratinolytic bacterium, Bacillus sp. MG-MASC-BT was isolated from feather dumping soil in Mallasamudram, Tamil Nadu, India and screened for keratinase production using alkali treated horn waste (HW) and feather waste (FW). The study factors influencing keratinase production was optimized by Box-Behnken design (BBD). The maximum enzyme production (1075. U/ml) was observed at pH 7.0, temperature 55. °C and growth period of 60. h. The media supplemented with 6\% of HW and FW enhanced keratinase production. Statistics based contour plots were generated to evaluate the changes in the response surface and to understand the relationship between the enzyme yield and the culture conditions.",

keywords = "Bacillus sp, Feather, Horn, Keratinase, Solid state fermentation",

author = "Muthusamy Govarthanan and Thangasamy Selvankumar and Kandasamy Selvam and Chinnappan Sudhakar and Vincent Aroulmoji and Seralathan Kamala-Kannan",

note = "Publisher Copyright: {\textcopyright} 2014 The Korean Society of Industrial and Engineering Chemistry.",

year = "2015",

month = jul,

day = "25",

doi = "10.1016/j.jiec.2014.12.022",

language = "English",

volume = "27",

pages = "25--30",

journal = "Journal of Industrial and Engineering Chemistry",

issn = "1226-086X",

}

Response surface methodology based optimization of keratinase production from alkali-treated feather waste and horn waste using Bacillus sp. MG-MASC-BT. / Govarthanan, Muthusamy; Selvankumar, Thangasamy; Selvam, Kandasamy et al.
In: Journal of Industrial and Engineering Chemistry, Vol. 27, 25.07.2015, p. 25-30.

Research output: Contribution to journal › Journal article › peer-review

TY - JOUR

T1 - Response surface methodology based optimization of keratinase production from alkali-treated feather waste and horn waste using Bacillus sp. MG-MASC-BT

AU - Govarthanan, Muthusamy

AU - Selvankumar, Thangasamy

AU - Selvam, Kandasamy

AU - Sudhakar, Chinnappan

AU - Aroulmoji, Vincent

AU - Kamala-Kannan, Seralathan

PY - 2015/7/25

Y1 - 2015/7/25

N2 - A native feather-degrading keratinolytic bacterium, Bacillus sp. MG-MASC-BT was isolated from feather dumping soil in Mallasamudram, Tamil Nadu, India and screened for keratinase production using alkali treated horn waste (HW) and feather waste (FW). The study factors influencing keratinase production was optimized by Box-Behnken design (BBD). The maximum enzyme production (1075. U/ml) was observed at pH 7.0, temperature 55. °C and growth period of 60. h. The media supplemented with 6% of HW and FW enhanced keratinase production. Statistics based contour plots were generated to evaluate the changes in the response surface and to understand the relationship between the enzyme yield and the culture conditions.

AB - A native feather-degrading keratinolytic bacterium, Bacillus sp. MG-MASC-BT was isolated from feather dumping soil in Mallasamudram, Tamil Nadu, India and screened for keratinase production using alkali treated horn waste (HW) and feather waste (FW). The study factors influencing keratinase production was optimized by Box-Behnken design (BBD). The maximum enzyme production (1075. U/ml) was observed at pH 7.0, temperature 55. °C and growth period of 60. h. The media supplemented with 6% of HW and FW enhanced keratinase production. Statistics based contour plots were generated to evaluate the changes in the response surface and to understand the relationship between the enzyme yield and the culture conditions.

KW - Bacillus sp

KW - Feather

KW - Horn

KW - Keratinase

KW - Solid state fermentation

UR - https://www.scopus.com/pages/publications/84930819624

U2 - 10.1016/j.jiec.2014.12.022

DO - 10.1016/j.jiec.2014.12.022

M3 - Journal article

AN - SCOPUS:84930819624

SN - 1226-086X

VL - 27

SP - 25

EP - 30

JO - Journal of Industrial and Engineering Chemistry

JF - Journal of Industrial and Engineering Chemistry

ER -

Response surface methodology based optimization of keratinase production from alkali-treated feather waste and horn waste using Bacillus sp. MG-MASC-BT

Abstract

Keywords

Quacquarelli Symonds(QS) Subject Topics

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