Role of thermophilic bacteria (Bacillus and Geobacillus) on crude oil degradation and biocorrosion in oil reservoir environment

Thermophilic bacterial communities generate thick biofilm on carbon steel API 5LX and produce extracellular metabolic products to accelerate the corrosion process in oil reservoirs. In the present study, nine thermophilic biocorrosive bacterial strains belonging to Bacillus and Geobacillus were isolated from the crude oil and produced water sample, and identified using 16S rRNA gene sequencing. The biodegradation efficiency of hydrocarbons was found to be high in the presence of bacterial isolates MN6 (82%), IR4 (94%) and IR2 (87%). During the biodegradation process, induction of the catabolic enzymes such as alkane hydroxylase, alcohol dehydrogenase and lipase were also examined in these isolates. Among them, the highest activity of alkane hydroxylase (130 µmol mg ⁻¹ protein) in IR4, alcohol dehydrogenase (70 µmol mg ⁻¹ protein) in IR2, and higher lipase activity in IR4 (60 µmol mg ⁻¹ protein) was observed. Electrochemical impedance spectroscopy and X-ray diffraction data showed that these isolates oxidize iron into ferrous/ferric oxides as the corrosion products on the carbon steel surface, whilst the crude oil hydrocarbon served as a sole carbon source for bacterial growth and development in such extreme environments.