Simple Fluorescence Turn-On Chemosensor for Selective Detection of Ba²⁺ Ion and Its Live Cell Imaging

A phenoxazine-based fluorescence chemosensor 4PB [(4-(tert-butyl)-N-(4-((4-((5-oxo-5H-benzo[a]phenoxazin-6-yl)amino)phenyl)sulfonyl)phenyl)benzamide)] was designed and synthesized by a simple synthetic methods. The 4PB fluorescence chemosensor selectively detects Ba²⁺ in the existence of other alkaline metal ions. In addition, 4PB showed high selectivity and sensitivity for Ba²⁺ detection. The detection limit of 4PB was 0.282 μM and the binding constant was 1.0 × 10⁶ M^-1 in CH₃CN/H₂O (97.5:2.5 v/v, HEPES = 1.25 mM, pH 7.3) medium. This chemosensor functioned through the intramolecular charge transfer (ICT) mechanism, which was further confirmed by DFT studies. Live cell imaging in MCF-7 cells confirmed the cell permeability of 4PB and its capability for specific detection of Ba²⁺ in living cells.