Site-specific incorporation of multiple units of functional nucleotides into DNA using a step-wise approach with polymerase and its application to monitoring DNA structural changes

Binh Huy Le; Van Thang Nguyen; Young Jun Seo

doi:10.1039/C8CC09444F

Site-specific incorporation of multiple units of functional nucleotides into DNA using a step-wise approach with polymerase and its application to monitoring DNA structural changes

Binh Huy Le
, Van Thang Nguyen
, Young Jun Seo^*

^*Corresponding author for this work

Department of Chemistry

Jeonbuk National University

Research output: Contribution to journal › Journal article › peer-review

8 Scopus citations

Abstract

We have developed a new method, a step-wise approach with polymerase, for site-specific incorporation of multiple units of functional nucleotides into DNA to form hairpin secondary structures. The fluorescence of the resulting DNA incorporating the functional nucleotides varied upon transitioning from single-strand to hairpin and duplex structures.

Original language	English
Pages (from-to)	2158-2161
Number of pages	4
Journal	Chemical Communications
Volume	55
Issue number	15
DOIs	https://doi.org/10.1039/C8CC09444F
State	Published - 2019

Quacquarelli Symonds(QS) Subject Topics

Materials Science
Engineering - Petroleum
Engineering - Chemical
Chemistry

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10.1039/C8CC09444F

Cite this

@article{a71fe6613cf24621bcf3bcc24f462eff,

title = "Site-specific incorporation of multiple units of functional nucleotides into DNA using a step-wise approach with polymerase and its application to monitoring DNA structural changes",

abstract = "We have developed a new method, a step-wise approach with polymerase, for site-specific incorporation of multiple units of functional nucleotides into DNA to form hairpin secondary structures. The fluorescence of the resulting DNA incorporating the functional nucleotides varied upon transitioning from single-strand to hairpin and duplex structures.",

author = "\{Huy Le\}, Binh and Nguyen, \{Van Thang\} and Seo, \{Young Jun\}",

note = "Publisher Copyright: {\textcopyright} The Royal Society of Chemistry.",

year = "2019",

doi = "10.1039/C8CC09444F",

language = "English",

volume = "55",

pages = "2158--2161",

journal = "Chemical Communications",

issn = "1359-7345",

number = "15",

}

Site-specific incorporation of multiple units of functional nucleotides into DNA using a step-wise approach with polymerase and its application to monitoring DNA structural changes. / Huy Le, Binh; Nguyen, Van Thang; Seo, Young Jun.
In: Chemical Communications, Vol. 55, No. 15, 2019, p. 2158-2161.

Research output: Contribution to journal › Journal article › peer-review

TY - JOUR

T1 - Site-specific incorporation of multiple units of functional nucleotides into DNA using a step-wise approach with polymerase and its application to monitoring DNA structural changes

AU - Huy Le, Binh

AU - Nguyen, Van Thang

AU - Seo, Young Jun

N1 - Publisher Copyright: © The Royal Society of Chemistry.

PY - 2019

Y1 - 2019

N2 - We have developed a new method, a step-wise approach with polymerase, for site-specific incorporation of multiple units of functional nucleotides into DNA to form hairpin secondary structures. The fluorescence of the resulting DNA incorporating the functional nucleotides varied upon transitioning from single-strand to hairpin and duplex structures.

AB - We have developed a new method, a step-wise approach with polymerase, for site-specific incorporation of multiple units of functional nucleotides into DNA to form hairpin secondary structures. The fluorescence of the resulting DNA incorporating the functional nucleotides varied upon transitioning from single-strand to hairpin and duplex structures.

UR - https://www.scopus.com/pages/publications/85061607434

U2 - 10.1039/C8CC09444F

DO - 10.1039/C8CC09444F

M3 - Journal article

C2 - 30675606

AN - SCOPUS:85061607434

SN - 1359-7345

VL - 55

SP - 2158

EP - 2161

JO - Chemical Communications

JF - Chemical Communications

IS - 15

ER -

Site-specific incorporation of multiple units of functional nucleotides into DNA using a step-wise approach with polymerase and its application to monitoring DNA structural changes

Abstract

Quacquarelli Symonds(QS) Subject Topics

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