SIVmac Gag p27 capsid protein gene expression in potato

Tae Geum Kim; Ruth Ruprecht; William H.R. Langridge

doi:10.1016/j.pep.2004.04.015

SIV_mac Gag p27 capsid protein gene expression in potato

Tae Geum Kim
, Ruth Ruprecht
, William H.R. Langridge^*

^*Corresponding author for this work

Department of Bio-Convergence Science

Loma Linda University Health
Harvard University

Research output: Contribution to journal › Journal article › peer-review

7 Scopus citations

Abstract

A cDNA encoding the Simian immunodeficiency virus type (SIV_mac) Gag capsid protein was introduced into Solanum tuberosum cells by Agrobacterium tumefaciens-mediated transformation methods. The gag gene was detected in the genomic DNA of transformed leaf tissues by PCR DNA amplification. Immunoblot analysis of transformed potato plant extracts with anti-Gag monoclonal antibody showed that biologically active Gag protein was synthesized in transformed tuber tissues. Based on ELISA results, recombinant Gag protein made up 0.006-0.014% of total soluble tuber protein. The synthesis of SIV Gag in transformed potato tubers opens the way for development of Gag-based edible plant vaccines for protection against SIV and potentially HIV-1 infection.

Original language	English
Pages (from-to)	312-317
Number of pages	6
Journal	Protein Expression and Purification
Volume	36
Issue number	2
DOIs	https://doi.org/10.1016/j.pep.2004.04.015
State	Published - 2004.08

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Edible vaccine
HIV
Immune response
SIV Gag
Solanum tuberosum

Access to Document

10.1016/j.pep.2004.04.015

Cite this

@article{4880e5b78acd4a2d9e2e939484adcf63,

title = "SIVmac Gag p27 capsid protein gene expression in potato",

abstract = "A cDNA encoding the Simian immunodeficiency virus type (SIVmac) Gag capsid protein was introduced into Solanum tuberosum cells by Agrobacterium tumefaciens-mediated transformation methods. The gag gene was detected in the genomic DNA of transformed leaf tissues by PCR DNA amplification. Immunoblot analysis of transformed potato plant extracts with anti-Gag monoclonal antibody showed that biologically active Gag protein was synthesized in transformed tuber tissues. Based on ELISA results, recombinant Gag protein made up 0.006-0.014\% of total soluble tuber protein. The synthesis of SIV Gag in transformed potato tubers opens the way for development of Gag-based edible plant vaccines for protection against SIV and potentially HIV-1 infection.",

keywords = "Edible vaccine, HIV, Immune response, SIV Gag, Solanum tuberosum",

author = "Kim, \{Tae Geum\} and Ruth Ruprecht and Langridge, \{William H.R.\}",

year = "2004",

month = aug,

doi = "10.1016/j.pep.2004.04.015",

language = "English",

volume = "36",

pages = "312--317",

journal = "Protein Expression and Purification",

issn = "1046-5928",

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T1 - SIVmac Gag p27 capsid protein gene expression in potato

AU - Kim, Tae Geum

AU - Ruprecht, Ruth

AU - Langridge, William H.R.

PY - 2004/8

Y1 - 2004/8

N2 - A cDNA encoding the Simian immunodeficiency virus type (SIVmac) Gag capsid protein was introduced into Solanum tuberosum cells by Agrobacterium tumefaciens-mediated transformation methods. The gag gene was detected in the genomic DNA of transformed leaf tissues by PCR DNA amplification. Immunoblot analysis of transformed potato plant extracts with anti-Gag monoclonal antibody showed that biologically active Gag protein was synthesized in transformed tuber tissues. Based on ELISA results, recombinant Gag protein made up 0.006-0.014% of total soluble tuber protein. The synthesis of SIV Gag in transformed potato tubers opens the way for development of Gag-based edible plant vaccines for protection against SIV and potentially HIV-1 infection.

AB - A cDNA encoding the Simian immunodeficiency virus type (SIVmac) Gag capsid protein was introduced into Solanum tuberosum cells by Agrobacterium tumefaciens-mediated transformation methods. The gag gene was detected in the genomic DNA of transformed leaf tissues by PCR DNA amplification. Immunoblot analysis of transformed potato plant extracts with anti-Gag monoclonal antibody showed that biologically active Gag protein was synthesized in transformed tuber tissues. Based on ELISA results, recombinant Gag protein made up 0.006-0.014% of total soluble tuber protein. The synthesis of SIV Gag in transformed potato tubers opens the way for development of Gag-based edible plant vaccines for protection against SIV and potentially HIV-1 infection.

KW - Edible vaccine

KW - HIV

KW - Immune response

KW - SIV Gag

KW - Solanum tuberosum

UR - https://www.scopus.com/pages/publications/3142645734

U2 - 10.1016/j.pep.2004.04.015

DO - 10.1016/j.pep.2004.04.015

M3 - Journal article

C2 - 15249055

AN - SCOPUS:3142645734

SN - 1046-5928

VL - 36

SP - 312

EP - 317

JO - Protein Expression and Purification

JF - Protein Expression and Purification

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ER -