Synthesis and characterization of a fluorescent adenosine derivative for detection of intermolecular RNA G-quadruplexes

In Sun Kim; Young Jun Seo

doi:10.1016/j.tetlet.2014.01.048

Synthesis and characterization of a fluorescent adenosine derivative for detection of intermolecular RNA G-quadruplexes

In Sun Kim
, Young Jun Seo^*

^*Corresponding author for this work

Department of Chemistry

Jeonbuk National University

Research output: Contribution to journal › Journal article › peer-review

19 Scopus citations

Abstract

We synthesized a fluorescent adenosine derivative, rA^py, as a probe to study RNA structural transitions, in particular the intermolecular G-quadruplex formation. rA^py was incorporated into the dangling positions of guanine-rich oligonucleotides, which under physiological conditions undergo π-stacking on top of each other exhibiting a strong emission signal in their G-quadruplex conformation, but not in their single-stranded state.

Original language	English
Pages (from-to)	1461-1463
Number of pages	3
Journal	Tetrahedron Letters
Volume	55
Issue number	8
DOIs	https://doi.org/10.1016/j.tetlet.2014.01.048
State	Published - 2014.02.19

Keywords

Fluorophore
G-quadruplex
Intermolecular
Oligonucleotide
RNA

Quacquarelli Symonds(QS) Subject Topics

Engineering - Petroleum
Pharmacy & Pharmacology
Chemistry
Biological Sciences

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10.1016/j.tetlet.2014.01.048

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title = "Synthesis and characterization of a fluorescent adenosine derivative for detection of intermolecular RNA G-quadruplexes",

abstract = "We synthesized a fluorescent adenosine derivative, rApy, as a probe to study RNA structural transitions, in particular the intermolecular G-quadruplex formation. rApy was incorporated into the dangling positions of guanine-rich oligonucleotides, which under physiological conditions undergo π-stacking on top of each other exhibiting a strong emission signal in their G-quadruplex conformation, but not in their single-stranded state.",

keywords = "Fluorophore, G-quadruplex, Intermolecular, Oligonucleotide, RNA",

author = "Kim, \{In Sun\} and Seo, \{Young Jun\}",

year = "2014",

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day = "19",

doi = "10.1016/j.tetlet.2014.01.048",

language = "English",

volume = "55",

pages = "1461--1463",

journal = "Tetrahedron Letters",

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TY - JOUR

T1 - Synthesis and characterization of a fluorescent adenosine derivative for detection of intermolecular RNA G-quadruplexes

AU - Kim, In Sun

AU - Seo, Young Jun

PY - 2014/2/19

Y1 - 2014/2/19

N2 - We synthesized a fluorescent adenosine derivative, rApy, as a probe to study RNA structural transitions, in particular the intermolecular G-quadruplex formation. rApy was incorporated into the dangling positions of guanine-rich oligonucleotides, which under physiological conditions undergo π-stacking on top of each other exhibiting a strong emission signal in their G-quadruplex conformation, but not in their single-stranded state.

AB - We synthesized a fluorescent adenosine derivative, rApy, as a probe to study RNA structural transitions, in particular the intermolecular G-quadruplex formation. rApy was incorporated into the dangling positions of guanine-rich oligonucleotides, which under physiological conditions undergo π-stacking on top of each other exhibiting a strong emission signal in their G-quadruplex conformation, but not in their single-stranded state.

KW - Fluorophore

KW - G-quadruplex

KW - Intermolecular

KW - Oligonucleotide

KW - RNA

UR - https://www.scopus.com/pages/publications/84893840800

U2 - 10.1016/j.tetlet.2014.01.048

DO - 10.1016/j.tetlet.2014.01.048

M3 - Journal article

AN - SCOPUS:84893840800

SN - 0040-4039

VL - 55

SP - 1461

EP - 1463

JO - Tetrahedron Letters

JF - Tetrahedron Letters

IS - 8

ER -

Synthesis and characterization of a fluorescent adenosine derivative for detection of intermolecular RNA G-quadruplexes

Abstract

Keywords

Quacquarelli Symonds(QS) Subject Topics

Access to Document

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