Synthesis, characterization and in vitro cytotoxicity of gold nanoparticles using cultural filtrate of low shear modeled microgravity and normal gravity cultured K. pneumoniae

The cultural filtrates of Klebsiella pneumoniae cultured under normal gravity and low shear modeled microgravity were potentially used to biosynthesize gold nanoparticles using the precursor gold III chloride trihydrate. The gold nanoparticles produced were spherical, of size between 16-36 and 24-50 nm with the cultural filtrates of normal and low shear modeled microgravity respectively. The characteristic plasmon resonance of gold nanoparticles occurred between 535-550 nm in the spectral analysis. XRD reflections (1 1 1), (2 0 0), (2 2 0), (3 1 1) indicate the face centered cubic and crystalline nature of the biosynthesized gold nanoparticles. The FTIR study proposes that proteins excreted into the cultural filtrate were involved in the synthesis and stabilization of gold nanoparticles. The in vitro cytotoxicity on 3T3L1, H9c2 and HepG2 cell lines showed the nontoxic and biocompatible nature of biosynthesized gold nanoparticles.