T7 exo-mediated FRET-breaking combined with DSN-RNAse-TdT for the detection of microRNA with ultrahigh signal-amplification

Van Thang Nguyen; Binh Huy Le; Young Jun Seo

doi:10.1039/c9an00303g

T7 exo-mediated FRET-breaking combined with DSN-RNAse-TdT for the detection of microRNA with ultrahigh signal-amplification

Van Thang Nguyen
, Binh Huy Le
, Young Jun Seo^*

^*Corresponding author for this work

Department of Chemistry

Jeonbuk National University

Research output: Contribution to journal › Journal article › peer-review

9 Scopus citations

Abstract

A DSN-RNAse-TdT-T7 exo probing system allows the detection of miRNA 21 with very high sensitivity (LOD = 2.57 fM) and selectivity - the result of (i) avoiding the false-positive signal from miRNA reacting with TdT polymerase and (ii) signal amplification occurring through a FRET-breaking mechanism involving T7 exo.

Original language	English
Pages (from-to)	3216-3220
Number of pages	5
Journal	Analyst
Volume	144
Issue number	10
DOIs	https://doi.org/10.1039/c9an00303g
State	Published - 2019.05.21

Quacquarelli Symonds(QS) Subject Topics

Environmental Sciences
Engineering - Petroleum
Chemistry
Biological Sciences

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10.1039/c9an00303g

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title = "T7 exo-mediated FRET-breaking combined with DSN-RNAse-TdT for the detection of microRNA with ultrahigh signal-amplification",

abstract = "A DSN-RNAse-TdT-T7 exo probing system allows the detection of miRNA 21 with very high sensitivity (LOD = 2.57 fM) and selectivity - the result of (i) avoiding the false-positive signal from miRNA reacting with TdT polymerase and (ii) signal amplification occurring through a FRET-breaking mechanism involving T7 exo.",

author = "Nguyen, \{Van Thang\} and Le, \{Binh Huy\} and Seo, \{Young Jun\}",

note = "Publisher Copyright: {\textcopyright} 2019 The Royal Society of Chemistry.",

year = "2019",

month = may,

day = "21",

doi = "10.1039/c9an00303g",

language = "English",

volume = "144",

pages = "3216--3220",

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AU - Nguyen, Van Thang

AU - Le, Binh Huy

AU - Seo, Young Jun

PY - 2019/5/21

Y1 - 2019/5/21

N2 - A DSN-RNAse-TdT-T7 exo probing system allows the detection of miRNA 21 with very high sensitivity (LOD = 2.57 fM) and selectivity - the result of (i) avoiding the false-positive signal from miRNA reacting with TdT polymerase and (ii) signal amplification occurring through a FRET-breaking mechanism involving T7 exo.

AB - A DSN-RNAse-TdT-T7 exo probing system allows the detection of miRNA 21 with very high sensitivity (LOD = 2.57 fM) and selectivity - the result of (i) avoiding the false-positive signal from miRNA reacting with TdT polymerase and (ii) signal amplification occurring through a FRET-breaking mechanism involving T7 exo.

UR - https://www.scopus.com/pages/publications/85065638014

U2 - 10.1039/c9an00303g

DO - 10.1039/c9an00303g

M3 - Journal article

C2 - 30984925

AN - SCOPUS:85065638014

SN - 0003-2654

VL - 144

SP - 3216

EP - 3220

JO - Analyst

JF - Analyst

IS - 10

ER -

T7 exo-mediated FRET-breaking combined with DSN-RNAse-TdT for the detection of microRNA with ultrahigh signal-amplification

Abstract

Quacquarelli Symonds(QS) Subject Topics

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