Temperature downshift increases recombinant cytokine titer in Streptomyces lividans fermentation

Soon Il Yun; Ahmad R.M. Yahya; Doug Cossar; William A. Anderson; Jeno M. Scharer; Murray Moo-Young

doi:10.1023/A:1013798129637

Temperature downshift increases recombinant cytokine titer in Streptomyces lividans fermentation

Soon Il Yun
, Ahmad R.M. Yahya
, Doug Cossar
, William A. Anderson^*
, Jeno M. Scharer
, Murray Moo-Young

^*Corresponding author for this work

Department of Food Science and Technology

University of Waterloo
Cangene Corporation

Research output: Contribution to journal › Journal article › peer-review

11 Scopus citations

Abstract

Recombinant human interleukin-3 (rhIL-3) production by a Streptomyces lividans strain is subject to severe proteolytic degradation. Human interleukin-3 (rhIL-3) concentration in the culture supernatant typically reaches 100-120 mg 1^-1 about 20 h after inoculation, but the titer drops rapidly as the result of proteolysis. Reducing the culture temperature at this point has a dramatic effect on product yield, increasing the final concentration in the supernatant at least two-fold.

Original language	English
Pages (from-to)	1903-1905
Number of pages	3
Journal	Biotechnology Letters
Volume	23
Issue number	23
DOIs	https://doi.org/10.1023/A:1013798129637
State	Published - 2001

Keywords

Fermentation
Interleukin-3
Proteases
Recombinant protein
Streptomyces

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title = "Temperature downshift increases recombinant cytokine titer in Streptomyces lividans fermentation",

abstract = "Recombinant human interleukin-3 (rhIL-3) production by a Streptomyces lividans strain is subject to severe proteolytic degradation. Human interleukin-3 (rhIL-3) concentration in the culture supernatant typically reaches 100-120 mg 1-1 about 20 h after inoculation, but the titer drops rapidly as the result of proteolysis. Reducing the culture temperature at this point has a dramatic effect on product yield, increasing the final concentration in the supernatant at least two-fold.",

keywords = "Fermentation, Interleukin-3, Proteases, Recombinant protein, Streptomyces",

author = "Yun, \{Soon Il\} and Yahya, \{Ahmad R.M.\} and Doug Cossar and Anderson, \{William A.\} and Scharer, \{Jeno M.\} and Murray Moo-Young",

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doi = "10.1023/A:1013798129637",

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T1 - Temperature downshift increases recombinant cytokine titer in Streptomyces lividans fermentation

AU - Yun, Soon Il

AU - Yahya, Ahmad R.M.

AU - Cossar, Doug

AU - Anderson, William A.

AU - Scharer, Jeno M.

AU - Moo-Young, Murray

PY - 2001

Y1 - 2001

N2 - Recombinant human interleukin-3 (rhIL-3) production by a Streptomyces lividans strain is subject to severe proteolytic degradation. Human interleukin-3 (rhIL-3) concentration in the culture supernatant typically reaches 100-120 mg 1-1 about 20 h after inoculation, but the titer drops rapidly as the result of proteolysis. Reducing the culture temperature at this point has a dramatic effect on product yield, increasing the final concentration in the supernatant at least two-fold.

AB - Recombinant human interleukin-3 (rhIL-3) production by a Streptomyces lividans strain is subject to severe proteolytic degradation. Human interleukin-3 (rhIL-3) concentration in the culture supernatant typically reaches 100-120 mg 1-1 about 20 h after inoculation, but the titer drops rapidly as the result of proteolysis. Reducing the culture temperature at this point has a dramatic effect on product yield, increasing the final concentration in the supernatant at least two-fold.

KW - Fermentation

KW - Interleukin-3

KW - Proteases

KW - Recombinant protein

KW - Streptomyces

UR - https://www.scopus.com/pages/publications/0035210377

U2 - 10.1023/A:1013798129637

DO - 10.1023/A:1013798129637

M3 - Journal article

AN - SCOPUS:0035210377

SN - 0141-5492

VL - 23

SP - 1903

EP - 1905

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 23

ER -

Temperature downshift increases recombinant cytokine titer in Streptomyces lividans fermentation

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Keywords

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