The feline herpesvirus type 1 ICP4 down-regulates feline immunodeficiency virus long terminal repeat(LTR)-directed gene expression via the C/EBP site in the LTR

Yasushi Kawaguchi; Ken Maeda; Marcelo R. Pecoraro; Yasuo Inoshima; Hyung kwan Jang; Mariko Kohmoto; Kiyoko Iwatsuki; Yasuhiro Ikeda; Masahiro Shimojima; Yukinobu Tohya; Chieko Kai; Takeshi Mikami

doi:10.1292/jvms.57.1129

The feline herpesvirus type 1 ICP4 down-regulates feline immunodeficiency virus long terminal repeat(LTR)-directed gene expression via the C/EBP site in the LTR

Yasushi Kawaguchi
, Ken Maeda
, Marcelo R. Pecoraro
, Yasuo Inoshima
, Hyung kwan Jang
, Mariko Kohmoto
, Kiyoko Iwatsuki
, Yasuhiro Ikeda
, Masahiro Shimojima
, Yukinobu Tohya
, Chieko Kai
, Takeshi Mikami

Department of Veterinary Medicine

The University of Tokyo

Research output: Contribution to journal › Journal article › peer-review

1 Scopus citations

Abstract

We investigated effects of feline herpesvirus type 1 (FHV-1) ICP4 on feline immunodeficiency virus (FIV) long terminal repeat (LTR)-directed gene expression by transient transfection assay in Crandell feline kidney cells. We demonstrated that FHV-1 ICP4 significantly stimulates the FIV LTR after introduction of site-specific mutation of the C/EBP site in the LTR, and the C/EBP site is sufficient to confer inhibitory effects by FHV-1 ICP4 on a heterologous promoter. These results indicate that FHV-1 ICP4 possesses both ability to transactivate FIV LTR-directed gene expression and to down-regulate the FIV LTR via the C/EBP site.

Original language	English
Pages (from-to)	1129-1131
Number of pages	3
Journal	Journal of Veterinary Medical Science
Volume	57
Issue number	6
DOIs	https://doi.org/10.1292/jvms.57.1129
State	Published - 1995

Keywords

C/EBP site
FHV-1 ICP4
FIV LTR

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10.1292/jvms.57.1129

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Kawaguchi, Y., Maeda, K., Pecoraro, M. R., Inoshima, Y., Jang, H. K., Kohmoto, M., Iwatsuki, K., Ikeda, Y., Shimojima, M., Tohya, Y., Kai, C., & Mikami, T. (1995). The feline herpesvirus type 1 ICP4 down-regulates feline immunodeficiency virus long terminal repeat(LTR)-directed gene expression via the C/EBP site in the LTR. Journal of Veterinary Medical Science, 57(6), 1129-1131. https://doi.org/10.1292/jvms.57.1129

@article{41805ad30dc74aa5969ded082661ecb9,

title = "The feline herpesvirus type 1 ICP4 down-regulates feline immunodeficiency virus long terminal repeat(LTR)-directed gene expression via the C/EBP site in the LTR",

abstract = "We investigated effects of feline herpesvirus type 1 (FHV-1) ICP4 on feline immunodeficiency virus (FIV) long terminal repeat (LTR)-directed gene expression by transient transfection assay in Crandell feline kidney cells. We demonstrated that FHV-1 ICP4 significantly stimulates the FIV LTR after introduction of site-specific mutation of the C/EBP site in the LTR, and the C/EBP site is sufficient to confer inhibitory effects by FHV-1 ICP4 on a heterologous promoter. These results indicate that FHV-1 ICP4 possesses both ability to transactivate FIV LTR-directed gene expression and to down-regulate the FIV LTR via the C/EBP site.",

keywords = "C/EBP site, FHV-1 ICP4, FIV LTR",

author = "Yasushi Kawaguchi and Ken Maeda and Pecoraro, \{Marcelo R.\} and Yasuo Inoshima and Jang, \{Hyung kwan\} and Mariko Kohmoto and Kiyoko Iwatsuki and Yasuhiro Ikeda and Masahiro Shimojima and Yukinobu Tohya and Chieko Kai and Takeshi Mikami",

year = "1995",

doi = "10.1292/jvms.57.1129",

language = "English",

volume = "57",

pages = "1129--1131",

journal = "Journal of Veterinary Medical Science",

issn = "0916-7250",

number = "6",

}

Kawaguchi, Y, Maeda, K, Pecoraro, MR, Inoshima, Y, Jang, HK, Kohmoto, M, Iwatsuki, K, Ikeda, Y, Shimojima, M, Tohya, Y, Kai, C & Mikami, T 1995, 'The feline herpesvirus type 1 ICP4 down-regulates feline immunodeficiency virus long terminal repeat(LTR)-directed gene expression via the C/EBP site in the LTR', Journal of Veterinary Medical Science, vol. 57, no. 6, pp. 1129-1131. https://doi.org/10.1292/jvms.57.1129

The feline herpesvirus type 1 ICP4 down-regulates feline immunodeficiency virus long terminal repeat(LTR)-directed gene expression via the C/EBP site in the LTR. / Kawaguchi, Yasushi; Maeda, Ken; Pecoraro, Marcelo R. et al.
In: Journal of Veterinary Medical Science, Vol. 57, No. 6, 1995, p. 1129-1131.

Research output: Contribution to journal › Journal article › peer-review

TY - JOUR

T1 - The feline herpesvirus type 1 ICP4 down-regulates feline immunodeficiency virus long terminal repeat(LTR)-directed gene expression via the C/EBP site in the LTR

AU - Kawaguchi, Yasushi

AU - Maeda, Ken

AU - Pecoraro, Marcelo R.

AU - Inoshima, Yasuo

AU - Jang, Hyung kwan

AU - Kohmoto, Mariko

AU - Iwatsuki, Kiyoko

AU - Ikeda, Yasuhiro

AU - Shimojima, Masahiro

AU - Tohya, Yukinobu

AU - Kai, Chieko

AU - Mikami, Takeshi

PY - 1995

Y1 - 1995

N2 - We investigated effects of feline herpesvirus type 1 (FHV-1) ICP4 on feline immunodeficiency virus (FIV) long terminal repeat (LTR)-directed gene expression by transient transfection assay in Crandell feline kidney cells. We demonstrated that FHV-1 ICP4 significantly stimulates the FIV LTR after introduction of site-specific mutation of the C/EBP site in the LTR, and the C/EBP site is sufficient to confer inhibitory effects by FHV-1 ICP4 on a heterologous promoter. These results indicate that FHV-1 ICP4 possesses both ability to transactivate FIV LTR-directed gene expression and to down-regulate the FIV LTR via the C/EBP site.

AB - We investigated effects of feline herpesvirus type 1 (FHV-1) ICP4 on feline immunodeficiency virus (FIV) long terminal repeat (LTR)-directed gene expression by transient transfection assay in Crandell feline kidney cells. We demonstrated that FHV-1 ICP4 significantly stimulates the FIV LTR after introduction of site-specific mutation of the C/EBP site in the LTR, and the C/EBP site is sufficient to confer inhibitory effects by FHV-1 ICP4 on a heterologous promoter. These results indicate that FHV-1 ICP4 possesses both ability to transactivate FIV LTR-directed gene expression and to down-regulate the FIV LTR via the C/EBP site.

KW - C/EBP site

KW - FHV-1 ICP4

KW - FIV LTR

UR - https://www.scopus.com/pages/publications/0029449280

U2 - 10.1292/jvms.57.1129

DO - 10.1292/jvms.57.1129

M3 - Journal article

C2 - 8720064

AN - SCOPUS:0029449280

SN - 0916-7250

VL - 57

SP - 1129

EP - 1131

JO - Journal of Veterinary Medical Science

JF - Journal of Veterinary Medical Science

IS - 6

ER -

The feline herpesvirus type 1 ICP4 down-regulates feline immunodeficiency virus long terminal repeat(LTR)-directed gene expression via the C/EBP site in the LTR

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Keywords

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