The Induction of Heme Oxygenase-1 Modulates Bismuth Oxide-induced Cytotoxicity in Human Dental Pulp Cells

Kyung San Min; Hoon Sang Chang; Ji Myung Bae; Sang Hyuk Park; Chan Ui Hong; Eun Cheol Kim

doi:10.1016/j.joen.2007.07.012

The Induction of Heme Oxygenase-1 Modulates Bismuth Oxide-induced Cytotoxicity in Human Dental Pulp Cells

Kyung San Min
, Hoon Sang Chang
, Ji Myung Bae
, Sang Hyuk Park
, Chan Ui Hong
, Eun Cheol Kim^*

^*Corresponding author for this work

Department of Dentistry

Wonkwang University
Kyung Hee University
Dankook University

Research output: Contribution to journal › Journal article › peer-review

73 Scopus citations

Abstract

The aim of this study was to investigate the cytotoxic and nitric oxide (NO)-inducing effects of bismuth oxide (Bi₂O₃)-containing Portland cement (BPC) on human dental pulp cells. We also assessed whether heme oxygenase-1 (HO-1) is involved in BPC-induced cytotoxicity in dental pulp cells. Cytotoxicity and NO production induced by BPC were higher than those induced by Portland cement at 12 and 24 hours, and the former gradually decreased to the level observed for PC. HO-1 and inducible nitric oxide synthase messenger RNA expressions in the BPC group showed maximal increase at 24 hours, and it gradually decreased with increasing cultivation time. Hemin treatment reversed the BPC-induced cytotoxicity, whereas zinc protoporphyrin IX treatment increased the cytotoxicity. These results suggested that NO production by BPC correlates with HO-1 expression in dental pulp cells. Moreover, BPC-induced HO-1 expression in dental pulp cells plays a protective role against the cytotoxic effects of BPC.

Original language	English
Pages (from-to)	1342-1346
Number of pages	5
Journal	Journal of Endodontics
Volume	33
Issue number	11
DOIs	https://doi.org/10.1016/j.joen.2007.07.012
State	Published - 2007.11

Keywords

Bismuth oxide
cytotoxicity
nitric
Portland cement

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10.1016/j.joen.2007.07.012

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title = "The Induction of Heme Oxygenase-1 Modulates Bismuth Oxide-induced Cytotoxicity in Human Dental Pulp Cells",

abstract = "The aim of this study was to investigate the cytotoxic and nitric oxide (NO)-inducing effects of bismuth oxide (Bi2O3)-containing Portland cement (BPC) on human dental pulp cells. We also assessed whether heme oxygenase-1 (HO-1) is involved in BPC-induced cytotoxicity in dental pulp cells. Cytotoxicity and NO production induced by BPC were higher than those induced by Portland cement at 12 and 24 hours, and the former gradually decreased to the level observed for PC. HO-1 and inducible nitric oxide synthase messenger RNA expressions in the BPC group showed maximal increase at 24 hours, and it gradually decreased with increasing cultivation time. Hemin treatment reversed the BPC-induced cytotoxicity, whereas zinc protoporphyrin IX treatment increased the cytotoxicity. These results suggested that NO production by BPC correlates with HO-1 expression in dental pulp cells. Moreover, BPC-induced HO-1 expression in dental pulp cells plays a protective role against the cytotoxic effects of BPC.",

keywords = "Bismuth oxide, cytotoxicity, nitric, Portland cement",

author = "Min, \{Kyung San\} and Chang, \{Hoon Sang\} and Bae, \{Ji Myung\} and Park, \{Sang Hyuk\} and Hong, \{Chan Ui\} and Kim, \{Eun Cheol\}",

year = "2007",

month = nov,

doi = "10.1016/j.joen.2007.07.012",

language = "English",

volume = "33",

pages = "1342--1346",

journal = "Journal of Endodontics",

issn = "0099-2399",

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T1 - The Induction of Heme Oxygenase-1 Modulates Bismuth Oxide-induced Cytotoxicity in Human Dental Pulp Cells

AU - Min, Kyung San

AU - Chang, Hoon Sang

AU - Bae, Ji Myung

AU - Park, Sang Hyuk

AU - Hong, Chan Ui

AU - Kim, Eun Cheol

PY - 2007/11

Y1 - 2007/11

N2 - The aim of this study was to investigate the cytotoxic and nitric oxide (NO)-inducing effects of bismuth oxide (Bi2O3)-containing Portland cement (BPC) on human dental pulp cells. We also assessed whether heme oxygenase-1 (HO-1) is involved in BPC-induced cytotoxicity in dental pulp cells. Cytotoxicity and NO production induced by BPC were higher than those induced by Portland cement at 12 and 24 hours, and the former gradually decreased to the level observed for PC. HO-1 and inducible nitric oxide synthase messenger RNA expressions in the BPC group showed maximal increase at 24 hours, and it gradually decreased with increasing cultivation time. Hemin treatment reversed the BPC-induced cytotoxicity, whereas zinc protoporphyrin IX treatment increased the cytotoxicity. These results suggested that NO production by BPC correlates with HO-1 expression in dental pulp cells. Moreover, BPC-induced HO-1 expression in dental pulp cells plays a protective role against the cytotoxic effects of BPC.

AB - The aim of this study was to investigate the cytotoxic and nitric oxide (NO)-inducing effects of bismuth oxide (Bi2O3)-containing Portland cement (BPC) on human dental pulp cells. We also assessed whether heme oxygenase-1 (HO-1) is involved in BPC-induced cytotoxicity in dental pulp cells. Cytotoxicity and NO production induced by BPC were higher than those induced by Portland cement at 12 and 24 hours, and the former gradually decreased to the level observed for PC. HO-1 and inducible nitric oxide synthase messenger RNA expressions in the BPC group showed maximal increase at 24 hours, and it gradually decreased with increasing cultivation time. Hemin treatment reversed the BPC-induced cytotoxicity, whereas zinc protoporphyrin IX treatment increased the cytotoxicity. These results suggested that NO production by BPC correlates with HO-1 expression in dental pulp cells. Moreover, BPC-induced HO-1 expression in dental pulp cells plays a protective role against the cytotoxic effects of BPC.

KW - Bismuth oxide

KW - cytotoxicity

KW - nitric

KW - Portland cement

UR - https://www.scopus.com/pages/publications/35349031071

U2 - 10.1016/j.joen.2007.07.012

DO - 10.1016/j.joen.2007.07.012

M3 - Journal article

C2 - 17963960

AN - SCOPUS:35349031071

SN - 0099-2399

VL - 33

SP - 1342

EP - 1346

JO - Journal of Endodontics

JF - Journal of Endodontics

IS - 11

ER -

The Induction of Heme Oxygenase-1 Modulates Bismuth Oxide-induced Cytotoxicity in Human Dental Pulp Cells

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Keywords

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