The MicroRNA-92a/Sp1/MyoD Axis Regulates Hypoxic Stimulation of Myogenic Lineage Differentiation in Mouse Embryonic Stem Cells

Seo Yeon Lee; Jimin Yang; Jung Hwa Park; Hwa Kyoung Shin; Woo Jean Kim; Su Yeon Kim; Eun Ju Lee; Injoo Hwang; Choon Soo Lee; Jaewon Lee; Hyo Soo Kim

doi:10.1016/j.ymthe.2019.08.014

The MicroRNA-92a/Sp1/MyoD Axis Regulates Hypoxic Stimulation of Myogenic Lineage Differentiation in Mouse Embryonic Stem Cells

Seo Yeon Lee
, Jimin Yang
, Jung Hwa Park
, Hwa Kyoung Shin
, Woo Jean Kim
, Su Yeon Kim
, Eun Ju Lee
, Injoo Hwang
, Choon Soo Lee
, Jaewon Lee
, Hyo Soo Kim^*

^*Corresponding author for this work

Department of Molecular Biology

Seoul National University
Pusan National University
Kosin University

Research output: Contribution to journal › Journal article › peer-review

14 Scopus citations

Abstract

Lee et al. describe the enhanced differentiation of hypoxia-primed embryoid bodies (EBs) into the myogenic lineage. Interestingly, this process was not mediated by HIF1α or HIF2α, but rather by Sp1 induction, via binding to the MyoD promoter. Moreover, the transplantation of hypoxia-primed EBs was found to facilitate muscle regeneration in vivo, and this was also found to depend on Sp1. By analyzing miRNA expression profiles, the authors demonstrate that hypoxia induced Sp1 in EBs via suppression of miRNA-92a that was found to directly target the 3′ UTR of Sp1.

Original language	English
Pages (from-to)	142-156
Number of pages	15
Journal	Molecular Therapy
Volume	28
Issue number	1
DOIs	https://doi.org/10.1016/j.ymthe.2019.08.014
State	Published - 2020.01.8

Keywords

embryoid bodies
hypoxic microenvironment
microRNA
myogenic differentiation
transcription factor

Access to Document

10.1016/j.ymthe.2019.08.014

Cite this

@article{4fe74300f1ba401d8f219fbd151989aa,

title = "The MicroRNA-92a/Sp1/MyoD Axis Regulates Hypoxic Stimulation of Myogenic Lineage Differentiation in Mouse Embryonic Stem Cells",

abstract = "Lee et al. describe the enhanced differentiation of hypoxia-primed embryoid bodies (EBs) into the myogenic lineage. Interestingly, this process was not mediated by HIF1α or HIF2α, but rather by Sp1 induction, via binding to the MyoD promoter. Moreover, the transplantation of hypoxia-primed EBs was found to facilitate muscle regeneration in vivo, and this was also found to depend on Sp1. By analyzing miRNA expression profiles, the authors demonstrate that hypoxia induced Sp1 in EBs via suppression of miRNA-92a that was found to directly target the 3′ UTR of Sp1.",

keywords = "embryoid bodies, hypoxic microenvironment, microRNA, myogenic differentiation, transcription factor",

author = "Lee, \{Seo Yeon\} and Jimin Yang and Park, \{Jung Hwa\} and Shin, \{Hwa Kyoung\} and Kim, \{Woo Jean\} and Kim, \{Su Yeon\} and Lee, \{Eun Ju\} and Injoo Hwang and Lee, \{Choon Soo\} and Jaewon Lee and Kim, \{Hyo Soo\}",

note = "Publisher Copyright: {\textcopyright} 2019 The American Society of Gene and Cell Therapy",

year = "2020",

month = jan,

day = "8",

doi = "10.1016/j.ymthe.2019.08.014",

language = "English",

volume = "28",

pages = "142--156",

journal = "Molecular Therapy",

issn = "1525-0016",

number = "1",

}

TY - JOUR

T1 - The MicroRNA-92a/Sp1/MyoD Axis Regulates Hypoxic Stimulation of Myogenic Lineage Differentiation in Mouse Embryonic Stem Cells

AU - Lee, Seo Yeon

AU - Yang, Jimin

AU - Park, Jung Hwa

AU - Shin, Hwa Kyoung

AU - Kim, Woo Jean

AU - Kim, Su Yeon

AU - Lee, Eun Ju

AU - Hwang, Injoo

AU - Lee, Choon Soo

AU - Lee, Jaewon

AU - Kim, Hyo Soo

PY - 2020/1/8

Y1 - 2020/1/8

N2 - Lee et al. describe the enhanced differentiation of hypoxia-primed embryoid bodies (EBs) into the myogenic lineage. Interestingly, this process was not mediated by HIF1α or HIF2α, but rather by Sp1 induction, via binding to the MyoD promoter. Moreover, the transplantation of hypoxia-primed EBs was found to facilitate muscle regeneration in vivo, and this was also found to depend on Sp1. By analyzing miRNA expression profiles, the authors demonstrate that hypoxia induced Sp1 in EBs via suppression of miRNA-92a that was found to directly target the 3′ UTR of Sp1.

AB - Lee et al. describe the enhanced differentiation of hypoxia-primed embryoid bodies (EBs) into the myogenic lineage. Interestingly, this process was not mediated by HIF1α or HIF2α, but rather by Sp1 induction, via binding to the MyoD promoter. Moreover, the transplantation of hypoxia-primed EBs was found to facilitate muscle regeneration in vivo, and this was also found to depend on Sp1. By analyzing miRNA expression profiles, the authors demonstrate that hypoxia induced Sp1 in EBs via suppression of miRNA-92a that was found to directly target the 3′ UTR of Sp1.

KW - embryoid bodies

KW - hypoxic microenvironment

KW - microRNA

KW - myogenic differentiation

KW - transcription factor

UR - https://www.scopus.com/pages/publications/85073031842

U2 - 10.1016/j.ymthe.2019.08.014

DO - 10.1016/j.ymthe.2019.08.014

M3 - Journal article

C2 - 31606324

AN - SCOPUS:85073031842

SN - 1525-0016

VL - 28

SP - 142

EP - 156

JO - Molecular Therapy

JF - Molecular Therapy

IS - 1

ER -

The MicroRNA-92a/Sp1/MyoD Axis Regulates Hypoxic Stimulation of Myogenic Lineage Differentiation in Mouse Embryonic Stem Cells

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this