Tick troponin I-like molecule is a potent inhibitor for angiogenesis

Shinya Fukumoto; Takeshi Sakaguchi; Myungio You; Xuenan Xuan; Kozo Fujisaki

doi:10.1016/j.mvr.2006.02.003

Tick troponin I-like molecule is a potent inhibitor for angiogenesis

Shinya Fukumoto
, Takeshi Sakaguchi
, Myungio You
, Xuenan Xuan
, Kozo Fujisaki^*

^*Corresponding author for this work

Department of Veterinary Medicine

Research output: Contribution to journal › Journal article › peer-review

35 Scopus citations

Abstract

This is the first report identifying the anti-angiogenesis saliva molecule of the ixodid tick. In our previous study, we identified a troponin I-like molecule (HLTnI) of the ixodid hard tick Haemaphysalis longicornis, a vector for various pathogens. To investigate its potential inhibitory effects for angiogenesis, we expressed and purified recombinant HLTnI in Escherichia coli. In a vascular endothelial growth factor (VEGF) competitive angiogenesis assay, the recombinant HLTnI significantly inhibited the capillary formation of human vascular endothelial cells (HUVEC) in vitro. The inhibition was dose-dependent with an IC₅₀ of 18.95 nM. These results indicated that HLTnI is a potent angiogenesis inhibitor.

Original language	English
Pages (from-to)	218-221
Number of pages	4
Journal	Microvascular Research
Volume	71
Issue number	3
DOIs	https://doi.org/10.1016/j.mvr.2006.02.003
State	Published - 2006.05

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Angiogenesis
Haemaphysalis longicornis
Troponin I-like molecule
VEGF

Quacquarelli Symonds(QS) Subject Topics

Medicine
Biological Sciences

Access to Document

10.1016/j.mvr.2006.02.003

Cite this

@article{d881abbf96174ae0a1d554aa9d926513,

title = "Tick troponin I-like molecule is a potent inhibitor for angiogenesis",

abstract = "This is the first report identifying the anti-angiogenesis saliva molecule of the ixodid tick. In our previous study, we identified a troponin I-like molecule (HLTnI) of the ixodid hard tick Haemaphysalis longicornis, a vector for various pathogens. To investigate its potential inhibitory effects for angiogenesis, we expressed and purified recombinant HLTnI in Escherichia coli. In a vascular endothelial growth factor (VEGF) competitive angiogenesis assay, the recombinant HLTnI significantly inhibited the capillary formation of human vascular endothelial cells (HUVEC) in vitro. The inhibition was dose-dependent with an IC50 of 18.95 nM. These results indicated that HLTnI is a potent angiogenesis inhibitor.",

keywords = "Angiogenesis, Haemaphysalis longicornis, Troponin I-like molecule, VEGF",

author = "Shinya Fukumoto and Takeshi Sakaguchi and Myungio You and Xuenan Xuan and Kozo Fujisaki",

year = "2006",

month = may,

doi = "10.1016/j.mvr.2006.02.003",

language = "English",

volume = "71",

pages = "218--221",

journal = "Microvascular Research",

issn = "0026-2862",

number = "3",

}

TY - JOUR

T1 - Tick troponin I-like molecule is a potent inhibitor for angiogenesis

AU - Fukumoto, Shinya

AU - Sakaguchi, Takeshi

AU - You, Myungio

AU - Xuan, Xuenan

AU - Fujisaki, Kozo

PY - 2006/5

Y1 - 2006/5

N2 - This is the first report identifying the anti-angiogenesis saliva molecule of the ixodid tick. In our previous study, we identified a troponin I-like molecule (HLTnI) of the ixodid hard tick Haemaphysalis longicornis, a vector for various pathogens. To investigate its potential inhibitory effects for angiogenesis, we expressed and purified recombinant HLTnI in Escherichia coli. In a vascular endothelial growth factor (VEGF) competitive angiogenesis assay, the recombinant HLTnI significantly inhibited the capillary formation of human vascular endothelial cells (HUVEC) in vitro. The inhibition was dose-dependent with an IC50 of 18.95 nM. These results indicated that HLTnI is a potent angiogenesis inhibitor.

AB - This is the first report identifying the anti-angiogenesis saliva molecule of the ixodid tick. In our previous study, we identified a troponin I-like molecule (HLTnI) of the ixodid hard tick Haemaphysalis longicornis, a vector for various pathogens. To investigate its potential inhibitory effects for angiogenesis, we expressed and purified recombinant HLTnI in Escherichia coli. In a vascular endothelial growth factor (VEGF) competitive angiogenesis assay, the recombinant HLTnI significantly inhibited the capillary formation of human vascular endothelial cells (HUVEC) in vitro. The inhibition was dose-dependent with an IC50 of 18.95 nM. These results indicated that HLTnI is a potent angiogenesis inhibitor.

KW - Angiogenesis

KW - Haemaphysalis longicornis

KW - Troponin I-like molecule

KW - VEGF

UR - https://www.scopus.com/pages/publications/33744523329

U2 - 10.1016/j.mvr.2006.02.003

DO - 10.1016/j.mvr.2006.02.003

M3 - Journal article

C2 - 16631826

AN - SCOPUS:33744523329

SN - 0026-2862

VL - 71

SP - 218

EP - 221

JO - Microvascular Research

JF - Microvascular Research

IS - 3

ER -

Tick troponin I-like molecule is a potent inhibitor for angiogenesis

Abstract

UN SDGs

Keywords

Quacquarelli Symonds(QS) Subject Topics

Access to Document

Other files and links

Fingerprint

Cite this