TiO₂ nanorods via one-step electrospinning technique: A novel nanomatrix for mouse myoblasts adhesion and propagation

This study was aimed at the synthesis and characterization of novel Titania nanorods by sol-gel electrospinning technique. The physicochemical properties of the synthesized nanorods were determined by field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), and X-ray diffraction (XRD) pattern. To examine the in vitro cytotoxicity, mouse myoblast C2C12 cells were treated with different concentrations of as prepared TiO₂ nanorods and the viability of cells was analyzed by Cell Counting Kit-8 assay at regular time intervals. The morphological features of the cells attached with nanorods were examined by Bio-SEM. Cytotoxicity experiments indicated that the mouse myoblast cells could attach to the TiO₂ nanorods after being cultured. We observed that TiO₂ nanorods could support cell adhesion and growth and guide spreading behavior of myoblasts. We conclude that the electrospun TiO₂ nanorods scaffolds with unique morphology had excellent biocompatibility. Thus, the current work demonstrates that the as-synthesized TiO₂ nanorods represent a promising biomaterial to be exploited for various tissue engineering applications.