Ultra-High Efficient Colony PCR for High Throughput Screening of Bacterial Genes

Mohammad Abu Hena Mostofa Jamal; Satya Priya Sharma; Hea Jong Chung; Hyeon Jin Kim; Seong Tshool Hong; Seungkoo Lee

doi:10.1007/s12088-017-0665-1

Ultra-High Efficient Colony PCR for High Throughput Screening of Bacterial Genes

Mohammad Abu Hena Mostofa Jamal
, Satya Priya Sharma
, Hea Jong Chung
, Hyeon Jin Kim
, Seong Tshool Hong
, Seungkoo Lee^*

^*Corresponding author for this work

Department of Medicine

Jeonbuk National University
Islamic University, Kushtia
Seonam University
Jinis Biopharmaceuticals Inc.
Kangwon National University

Research output: Contribution to journal › Journal article › peer-review

15 Scopus citations

Abstract

Current colony PCR methods are not suitable for screening genes encoded in genomic DNA and are limited to E. coli host strains. Here, we describe an ultra-high efficient colony PCR method for high throughput screening of bacterial genes embedded in the genomic DNA of any bacterial species. This new technique expands colony PCR method to several hosts as well as offers a rapid, less expensive and reliable bacterial genomic DNA extraction.

Original language	English
Pages (from-to)	365-369
Number of pages	5
Journal	Indian Journal of Microbiology
Volume	57
Issue number	3
DOIs	https://doi.org/10.1007/s12088-017-0665-1
State	Published - 2017.09.1

Keywords

Colony PCR
DNA extraction
High throughput screening
Sequencing
Sonication

Quacquarelli Symonds(QS) Subject Topics

Biological Sciences

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10.1007/s12088-017-0665-1

Cite this

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title = "Ultra-High Efficient Colony PCR for High Throughput Screening of Bacterial Genes",

abstract = "Current colony PCR methods are not suitable for screening genes encoded in genomic DNA and are limited to E. coli host strains. Here, we describe an ultra-high efficient colony PCR method for high throughput screening of bacterial genes embedded in the genomic DNA of any bacterial species. This new technique expands colony PCR method to several hosts as well as offers a rapid, less expensive and reliable bacterial genomic DNA extraction.",

keywords = "Colony PCR, DNA extraction, High throughput screening, Sequencing, Sonication",

author = "Jamal, \{Mohammad Abu Hena Mostofa\} and Sharma, \{Satya Priya\} and Chung, \{Hea Jong\} and Kim, \{Hyeon Jin\} and Hong, \{Seong Tshool\} and Seungkoo Lee",

note = "Publisher Copyright: {\textcopyright} 2017, Association of Microbiologists of India.",

year = "2017",

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doi = "10.1007/s12088-017-0665-1",

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TY - JOUR

T1 - Ultra-High Efficient Colony PCR for High Throughput Screening of Bacterial Genes

AU - Jamal, Mohammad Abu Hena Mostofa

AU - Sharma, Satya Priya

AU - Chung, Hea Jong

AU - Kim, Hyeon Jin

AU - Hong, Seong Tshool

AU - Lee, Seungkoo

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Current colony PCR methods are not suitable for screening genes encoded in genomic DNA and are limited to E. coli host strains. Here, we describe an ultra-high efficient colony PCR method for high throughput screening of bacterial genes embedded in the genomic DNA of any bacterial species. This new technique expands colony PCR method to several hosts as well as offers a rapid, less expensive and reliable bacterial genomic DNA extraction.

AB - Current colony PCR methods are not suitable for screening genes encoded in genomic DNA and are limited to E. coli host strains. Here, we describe an ultra-high efficient colony PCR method for high throughput screening of bacterial genes embedded in the genomic DNA of any bacterial species. This new technique expands colony PCR method to several hosts as well as offers a rapid, less expensive and reliable bacterial genomic DNA extraction.

KW - Colony PCR

KW - DNA extraction

KW - High throughput screening

KW - Sequencing

KW - Sonication

UR - https://www.scopus.com/pages/publications/85027131619

U2 - 10.1007/s12088-017-0665-1

DO - 10.1007/s12088-017-0665-1

M3 - Journal article

AN - SCOPUS:85027131619

SN - 0046-8991

VL - 57

SP - 365

EP - 369

JO - Indian Journal of Microbiology

JF - Indian Journal of Microbiology

IS - 3

ER -

Ultra-High Efficient Colony PCR for High Throughput Screening of Bacterial Genes

Abstract

Keywords

Quacquarelli Symonds(QS) Subject Topics

Access to Document

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