Abstract
Abstract Thrombin binding G-quadruplex oligonucleotide containing two TT-dimer fragments and a gold attachment (ODN G1-G) was designed and synthesized with the aim of understanding the TT-dimer effect in G-quadruplex formation. Our results showed that TT-dimer mutation induced by UV light inhibits the formation of and even destroys the G-quadruplex structure, as confirmed by UV, CD and melting temperature measurements. The structural change resulting from TT-dimer formation with DNA was additionally probed and was found to be accompanied by significant gold aggregation that was observed in the form of a signal change from red to blue.
| Original language | English |
|---|---|
| Article number | 22549 |
| Pages (from-to) | 2434-2437 |
| Number of pages | 4 |
| Journal | Bioorganic and Medicinal Chemistry Letters |
| Volume | 25 |
| Issue number | 11 |
| DOIs | |
| State | Published - 2015.06.1 |
Keywords
- Fluorophore
- G-quadruplex structure
- Gold aggregation
- Probe
- TT-dimerization
Quacquarelli Symonds(QS) Subject Topics
- Medicine
- Engineering - Petroleum
- Pharmacy & Pharmacology
- Chemistry
- Biological Sciences
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